Restriction enzyme BstZ17I is sensitive to cytosine methylation
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چکیده
منابع مشابه
Restriction enzyme HincII is sensitive to methylation of cytosine that occurs 5' to the recognition sequence.
In this paper we demonstrate that the HincII restriction endonuclease, in addition to being sensitive to methylation of the 3′ A and C residues, is also sensitive to methylation of a cytosine immediately 5′ to the recognition sequence. Having encountered this property in one of the sites in the mouse c-fos gene, we confirmed the sensitivity of HincII to the 5′ cytosine methylation in in vitro m...
متن کاملThe restriction enzyme EheI (GGC/GCC) is sensitive to CpG methylation.
Ehel cleaves the site GGC/GCC and is an isoschizomer of Narl (GG/CGCQ and Bbel (GGCGC/C) (1 -4) . Here I show that Ehel is methyl sensitive and will not cleave its CpG methylated site (GC^CGCC). Narl and Bbel are also CpG methyl sensitive (5—7). Ehel methyl sensitivity is shown by digesting mixtures of methylated X DNA and unmethylated plasmid DNA and viceversa (Figure 1). I also digest eukaryo...
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Methylation of cytosine to 5-methylcytosine (mC) is the most important epigenetic DNA alteration in eukaryotes. Cytosine methylation is involved in establishing a silenced chromatin stage through interaction with DNA-binding proteins and recruitment of histone deacetylases and other histone-modifying enzymes leading to chromatin remodeling. In this fashion, DNA methylation is connected with pro...
متن کاملThe restriction enzyme AlwNI is blocked by overlapping methylation.
Most strains of E.coli contain two sequence specific methylases, Dam and Dem. The adenine residue of the sequence GATC is methylated at the N 6 position by the Dam methylase whereas the Dem methylase recognizes the sequences CC(A/T)GG methylating the internal cytosine at the C 5 position. The commercially available restriction enzyme AlwNl (New England Biolabs) has a recognition sequence CAGN 3...
متن کاملCytosine Methylation Dysregulation in Neonates Following Intrauterine Growth Restriction
BACKGROUND Perturbations of the intrauterine environment can affect fetal development during critical periods of plasticity, and can increase susceptibility to a number of age-related diseases (e.g., type 2 diabetes mellitus; T2DM), manifesting as late as decades later. We hypothesized that this biological memory is mediated by permanent alterations of the epigenome in stem cell populations, an...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2001
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(01)00227-0